Pre-activated NHS resin functionalized on a modern, high flow agarose base matrix. To Sepharose 4 Fast Flow (90 µm) and Sepharose 6 Fast Flow. GE Healthcare NHS-Activated Sepharose™ 4 Fast Flow Affinity Media. Use for coupling amino-containing smaller proteins and peptides. GE Healthcare Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH. Product Code.